Is the file hosted from another computer or local to your machine?
My database files are hosted on FM Server.
Try taking the file down off the server, make your printer setup choices that you want for that layout (Try it while in layout mode...) Then put it back up on the server and test.
I have found that not all printer setup option are retained so you may need to use a script to specify these options. (And custom page sizes seem to need to be manually selected by the user as even the script can't seem to select them in one of my systems used with windows ...)